Report for the period 1 April 2026 to 30 June 2026.

Between 1 April and 30 June 2026, research work continued on project activities 2.d1, 2d.2, 2d.3 and 2d.6

Between April and the end of June 2026, as part of Activity 2.d1, work continued on the development of prebiotic extracts containing XOS and the evaluation of their applications, with particular attention paid to improving the organoleptic and technological properties of the products, the scalability of the process and the potential applications of the final product in food trials. Tests were carried out to improve the colour and flavour of the prebiotic extracts, and the potential uses of lignocellulose by-products were identified to broaden the scope of application for potential raw materials and intermediates within the context of the circular economy. Mass balance calculations for integrated prebiotic production were carried out, as well as economic modelling of the integrated prebiotic production process, assessing the material flows of the technological process, the production logic and the potential economic viability. Prebiotic extracts were prepared for food testing, whilst simultaneously carrying out an assessment of food labelling and dosage in accordance with the requirements of European Union regulations, to ensure that further testing and potential use of the product comply with the regulatory framework.  

As part of Activity 2d.2, research into marine protists continued Aurantiochytrium limacinum (Schizochytrium) changes in biomass composition under various cultivation conditions. Research has begun into the effect of ethanol on  Aurantiochytrium limacinum lipid biosynthesis was studied in cultures grown on hydrolysate of oat straw dust, and the lipid fatty acid profile was analysed.

As part of Activity 2d.3, research into microalgae continued Graesiella emersonii  changes in the composition of biomass under various cultivation conditions in oat straw dust hydrolysate. The dynamics of the cultivation process under mixotrophic and heterotrophic conditions were investigated.

As part of Activity 2d.6, research continued into the use of xylooligosaccharide (XOS)-containing hemicellulose hydrolysates as additives in the development of multifunctional products. Research has been initiated into the use of purified XOS extracts in formulations for multifunctional products.